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Northwest Missouri State University


Quantitative (Real Time) PCR (qPCR)

Quantitative (Real Time) PCR

qPCR is the method of choice to quantitatively measure starting amounts of DNA, cDNA or RNA. It is commonly used to determine whether a DNA sequence is present in a sample and the number of its copies in the sample. The method with currently the highest level of accuracy is Quantitative real-time PCR. It is also known as RT-PCR (Real Time PCR) or RQ-PCR.