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Chemiluminescence describes the emission of light and heat as the result of chemical reaction. Sometimes it is called as chemoluminescence. It is a form of electromagnetic radiation which just happens to fall in the visible region, making it easy to see and identify. Enhanced chemiluminescence is a common technique for a variety of detection assays in biology. Applications includes in pharmaceutical Industries (analysis and quality control), clinical sciences, alumina industries, forensic analysis, detecting the photoactivity of water through its H2O2 concentration, detecting nitric oxide in the breath of asthma patients.